鹌鹑SLC24A5基因多态性与羽色性状的关联性分析
China Poultry（2023）
河南科技大学
Abstract
为研究SLC24A5基因与鹌鹑羽色性状的关系,试验采用RT-qPCR方法研究朝鲜鹌鹑和北京白羽鹌鹑在胚胎不同发育时期的mRNA表达水平.结果显示:SLC24A5基因在朝鲜鹌鹑胚胎发育时期的表达水平极显著高于北京白羽鹌鹑(P<0.01);鲜鹌鹑和北京白羽鹌鹑的3种基因型频率分布差异极显著(P<0.01).位于外显子1的SNP1(c.A5T)和外显子6 的SNP2(c.G703A)均为中性位点,但SNP2(c.G703A)位点造成的编码蛋白G235S位点突变并不保守,属于多变性位点.研究表明,SLC24A5基因与鹌鹑羽色性状有关联性,可以为鹌鹑羽色育种提供理论依据.
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Key words
quail,feather color,SLC24A5 gene,gene mutation
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