上转发光免疫层析法快速检测棘球蚴病患者血清IgG的评价
Chinese Journal of Parasitology and Parasitic Diseases（2022）
Abstract
初步评价上转发光免疫层析(UPT-LF)法快速检测棘球蚴病患者血清IgG的效果,并与酶联免疫吸附试验(ELISA)法检测结果作比较.2017—2018年,从青海省人民医院和青海大学附属医院收集棘球蚴病临床确诊患者血清,同时从各医院体检中心收集健康者血清,以UPT-LF法和ELISA法检测血样中棘球蚴IgG抗体,以临床诊断结果为标准分析两种检测方法检测的灵敏度、特异度、误诊率、总符合率、约登指数等.两种检测结果的比较进行卡方检验.共收集棘球蚴病患者血样104份,其中多房棘球蚴病(AE)46份,细粒棘球蚴病(CE)58份;非棘球蚴病患者血样165份.UPT-LF、ELISA法检测104份临床确诊棘球蚴病患者血清的灵敏度分别为94.2％(98/104)、78.8％(82/104),二者差异有统计学意义(χ2=11.250,P<0.01),其中检测AE的灵敏度分别为97.8％(45/46)、89.1％(41/46),检测CE的灵敏度分别为91.4％(53/58)、70.7％(41/58);UPT-LF、ELISA法检测165份非棘球蚴病患者血清的特异度分别为95.2％(157/165)、90.9％(150/165),二者差异无统计学意义(χ2=1.565,P>0.05).UPT-LF、ELISA法检测结果与临床诊断结果的符合率分别为94.8％(255/269)、86.2％(232/269),二者差异无统计学意义(χ2=1.884,P>0.05).UPT-LF、ELISA法的约登指数分别为0.894、0.697.与ELISA法相比,UPT-LF法的灵敏度更高,具有良好的棘球蚴病现场快速检测应用前景.
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