The spatial-dimensional and temporal-dimensional fate of nanocarrier-loaded dissolving microneedles with different lengths of needles

•P4 probes with aggregation caused quenching property enabled the signal discrimination of integrated nanocarriers (NC) from disintegrated NC.•Clarify the effect of the height of dissolving microneedles (DMNs) on in vivo fate of NC loaded in DMNs from spatial and temporal perspectives.•Facilitate the clinical translation of NC-incorporated DMNs.