Extraction-free kit and method for detecting polymorphism of CYP2C19 gene
user-5f8411ab4c775e9685ff56d3（2018）
Abstract
The invention discloses an extraction-free kit for detecting the polymorphism of the CYP2C19 gene. The kit comprises a 2*PCR reaction solution, Oligo Mix A, Oligo Mix B, a DNA polymerase, a positive control and a negative control. The invention also discloses an extraction-free method for detecting the polymorphism of the CYP2C19 gene. The DNA polymerase is one or more selected from DNA polymerases that have strong resistance to PCR inhibitors by themselves or through genetic engineering. The kit and the detection method of the invention truly realize the direct detection of trace samples, andget rid of the step of DNA extraction from peripheral blood and swab samples. According to the invention, a trace amount of a blood sample or buccal swab sample is directly added into the PCR reaction solution, and a PCR amplification program is run to complete the detection of the polymorphism of the CYP2C19 gene. The whole detection process does not involve toxic reagents, so the kit and the detection method are safe, convenient, simple to operation, show in the whole detection time, high in sensitivity, good in specificity and applicable to high-throughput detection.
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Key words
DNA extraction,Buccal swab,Polymerase chain reaction,DNA polymerase,Polymerase,Gene,Polymorphism (computer science),A-DNA,Molecular biology,Chemistry
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