基于万寿菊转录组测序的SSR标记开发
Acta Horticulturae Sinica（2018）
Abstract
万寿菊(Tagetes erectaL.)花蕾转录组测序共获得48 953条Unigene,利用MISA软件检测出20 666个SSR位点,分布于13 849条Unigene中,出现频率为28.29％,平均分布距离为2.51 kb.优势重复基序为三核苷酸、四核苷酸,分别占总SSR位点的50.16％和20.94％.ATG/ATG和AAAC/GTTT分别是三核苷酸、四核苷酸的优势重复基元,占总SSR重复类型的13.82％和3.66％.随机选取不同主导重复基元类型并合成SSR引物36对,以20份万寿菊自交系的基因组DNA为模板,对引物有效性和多态性进行了验证,30对引物可以扩增到清晰稳定的目标条带,有效扩增率为80.56％;其中13对引物具有多态性,平均He和PIC分别为0.275和0.608.以上结果表明,万寿菊转录组测序产生的Unigene信息可作为开发SSR标记的有效来源,获得的大批量SSR标记可为万寿菊的遗传多样性分析和遗传图谱构建提供可靠的标记选择.
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Key words
Tagetes erecta L.,SSR,transcriptome
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