婴幼儿配方乳粉加工环境中蜡样芽孢杆菌多位点序列分型
Science and Technology of Food Industry（2018）
Abstract
以分离自婴幼儿配方乳粉加工环境中蜡样芽孢杆菌为研究对象,利用多位点序列分型(MLST)技术对蜡样芽孢杆菌的多样性和系统进化关系进行探究.结果表明,84株蜡样芽孢杆菌共分成24个ST型,分别是ST-24、ST-26、ST-32、ST-62、ST-144、ST-374、ST-999、ST-1119、ST-1243、ST-1284、ST-1333、ST-1334、ST-1335、ST-1336、ST-1337、ST-1338、ST-1339、ST-1340、ST-1341、ST-1342、ST-1343、ST-1344、ST-1345及ST-1347,其中ST-999(22.62％)、ST-1343(15.48％)、ST-1335(7.14％)与ST-1345(7.14％)是该婴幼儿配方乳粉加工环境中的优势ST型.同时发现了3个新的等位基因pur-242,pyc-198,ilv-276与14个新的ST型ST-1333、ST-1334、ST-1335、ST-1336、ST-1337、ST-1338、ST-1339、ST-1340、ST-1341、ST-1342、ST-1343、ST-1344、ST-1345和ST-1347.系统发育分析表明24个ST型与B.cereus、B.anthracis和B.thuringiensis这三个种显示了更近的系统发育关系,与蜡样芽孢杆菌群体中的另外8个种的亲缘关系较远.
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