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Optimized GMP-grade Production of Non-Viral Sleeping Beauty-generated CARCIK Cells for Enhanced Fitness and Clinical Scalability

Ilaria Pisani, Giusi Melita,Patricia Borges de Souza,Stefania Galimberti,Angela Maria Savino,Jolanda Sarno, Beatrice Landoni, Stefano Crippa,Elisa Gotti, Carolina Cuofano, Olga Pedrini,Chiara Capelli,Giada Matera, Daniela Belotti, Stefania Cesana,Benedetta Cabiati, Michele Quaroni, Valentina Colombo,Massimiliano Mazza,Barbara Vergani,Anna Gaimari,Fabio Nicolini,Marcella Tazzari, Martine Bocchini,Marta Serafini, Alessandro Rambaldi,Benedetta Rambaldi, Giuseppe Dastoli, Andrea Biondi,Giuseppe Gaipa,Martino Introna,Josée Golay,Sarah Tettamanti

Journal of Translational Medicine（2025）

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Key words
Good manufacturing practices (GMP),CAR T cells,Hematological malignancies,Sleeping beauty transposon,Workflow optimization,T cell fitness
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