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Comparative Evaluation of ACetic - MEthanol High Salt Dissociation Approach for Single-Cell Transcriptomics of Frozen Human Tissues

Marina Utkina,Anastasia Shcherbakova,Ruslan Deviatiiarov,Alina Ryabova, Marina Loguinova, Valentin Trofimov, Anna Kuznetsova, Mikhail Petropavlovskiy,Rustam Salimkhanov, Denis Maksimov, Eugene Albert,Alexandra Golubeva,Walaa Asaad, Lilia Urusova,Ekaterina Bondarenko,Anastasia Lapshina, Alexandra Shutova,Dmitry Beltsevich,Oleg Gusev,Larisa Dzeranova,Galina Melnichenko,Ildar Minniakhmetov,Ivan Dedov,Natalya Mokrysheva, Sergey Popov

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY（2025）

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Key words
ScRNA-seq,ACME HS,cryopreservation,dissociation,fresh-frozen tissue,fixed single cells,human endocrine glands
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