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Non-viral, High Throughput Genetic Engineering of Primary Immune Cells Using Nanostraw-Mediated Transfection

Arun R. K. Kumar,Jessalyn Low, Jet Lim,Ba Myint, Xinhong Sun, Ling Wu,Hong Sheng Cheng, Sophronia Yip, Cyrus Zai Ming Cheng, Thamizhanban Manoharan,Ying Jie Quek,Yufeng Shou,Johann Shane Tian,Yu Yang Ng, Nicholas R. J. Gascoigne,Nguan Soon Tan,Rio Sugimura,Gloryn Chia,Alice Man Sze Cheung, Makoto Yawata,Andy Tay

BIOMATERIALS（2025）

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Key words

Intracellular delivery,CAR-T,Nanostraws,CAR-Immune cells,CRISPR/Cas9 gene editing

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