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Assessment of Real-Time PCR Techniques for the Detection of Airborne Fungal Pathogens of Wheat: Role of DNA Extraction on Spore Quantification

Ilakkiya Thirugnanasambandam, Tara Vucurevich, Tara Shelton,Nat N. V. Kav,Claudia Sheedy,Andre Laroche, Jonathan K. Challis

ACS AGRICULTURAL SCIENCE & TECHNOLOGY（2024）

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关键词
RT-iPCR,DNA extractionefficiency,qPCR,fungal pathogens,sporequantification
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