FIGURE 4 from Small Extracellular Vesicles Promote Stiffness-mediated Metastasis

Stiff EVs promote cancer cell migration and dissemination. A, Schematic of 2-day GFP+ zebrafish embryo model system used to test the ability of MDA-MB-231 EVs (red, filled triangles) to disperse MDA-MB-231 cancer cells (blue, open triangles). Scale bar, 100 µm. B, Full zebrafish body images of the embryos at time 0 and 24 hours postinjection of MDA-MB-231 cancer cells. Scale bar, 100 µm. Representative images. C, Enlarged images of disseminated MDA-MB-231 cells (white) and EVs (red). Scale bar, 100 µm. Representative images. D, Percentage of injected embryos with cancer cell dissemination to the head or the tail. Total number of fish per condition is 47 for PBS control, 63 for 25 kPa, and 78 for 0.5 kPa condition. Five biological repeats of EVs. One-way ANOVA.