Development of a microsatellite marker resource for the thin-lipped mullet, Chelon ramada (Risso, 1827)

Khadidja Meziane, Bruno Guinand,Mohamed Hichem Kara,Lamya Chaoui

Biologia(2024)

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摘要
Thin-lipped mullet (Chelon ramada) is an important resource in the Mediterranean Sea and a candidate species for aquaculture. As microsatellite loci remain the markers of choice for the evaluation of breeding programmes or stock assessment, we hereby provide and share a resource of 3,883 species-specific microsatellite loci for this species, where they are currently lacking. This resource includes primer pairs useful for polymerase chain reaction (PCR) amplification. A subset of nine loci including di-, tri- and tetranucleotide loci with correct amplification success rates have been specifically tested under identical PCR conditions, opening up new avenues for research.
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关键词
Microsatellite,High-throughput sequencing,Mugilids,Mediterranean Sea,Resource sharing
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