Evaluation of non-invasive dsRNA delivery methods for the development of RNA interference in the Asian tiger mosquito Aedes albopictus

The Asian tiger mosquito Aedes albopictus is one of the most invasive species and an efficient vector of several pathogens. RNA interference (RNAi) has been proposed as an alternative method to control mosquito populations by silencing the expression of genes that are essential for their survival. However, the optimal delivery method for dsRNAs to enhance an optimal RNAi remains elusive and comparative studies are lacking. We have, therefore, compared the efficiency of three non-invasive delivery methods to mosquito larvae: soaking, rehydration and nanoparticle ingestion. Each method was tested separately on four genes predicted to code non-essential proteins (i.e., collagenase-like, kynurenine 3-monooxygenase-like, yellow-like and venom serine protease-like) in order to be able to compare the importance of gene knock-down. All tested methods successfully downregulated mosquito gene expression. However, silencing efficiency strongly varies among methods and genes. Silencing (95.1