Protocol for the acute electrical stimulation of thoracolumbar and sacral nerve roots and colonic motility measurements in anesthetized pigs v2

This protocol describes a process for the measurement of acute electrical stimulation-induced effects on colonic motility in anesthetized young adult Yucatan minipigs. Signals recorded from manometry probes inserted into the proximal, transverse and distal colonic regions were used to measure the effect of stimulation on the sacral or thoracolumbar nerve roots in an acute anesthetized preparation. The effect of stimulation was quantified as motility index/area under the curve (AUC) assessments before, during and after stimulation, and the data was used to create a functional map of colonic motor response to spinal nerve roots stimulation.