Supplementary Figures S1 - S11 from Human ASXL1-Mutant Hematopoiesis Is Driven by a Truncated Protein Associated with Aberrant Deubiquitination of H2AK119

Supplementary Figure 1: Overview of Stanford cohort. Supplementary Figure S2. Pooled screening of ASXL1 coding sequence. Supplementary Figure S3. Detection of truncated ASXL1 in CRISPR/Cas9-edited HSPCs. Supplementary Figure S4. Myeloid differentiation and in vitro proliferation assays. Supplementary Figure S5. Phenotyping of ASXL1 Trunc-derived myeloid malignancies. Supplementary Figure S6. H2AK119Ub in HSPC subsets. Supplementary Figure S7. Generation and phenotyping of corrected K562 cell line, BAP1 deletion and overexpression. Supplementary Figure S8. Consistent pattern of H2AK119Ub distribution dependent on PR-DUB activity. Supplementary Figure S9. Correlation of Gene Set enrichment with H2AK119Ub, ATAC-Seq and RNA-Seq. Supplementary Figure S10. Validation and phenotyping of inducible expression of truncated ASXL1 in K562 cells. Supplementary Figure S11. Inducible knockdown of RING1A and RING1B.