Efficient production of itaconic acid from the single carbon substrate methanol with engineered Komagataella phaffii
crossref(2024)
摘要
Background Amidst the escalating carbon dioxide levels resulting from fossil fuel consumption, there is a pressing need for sustainable, bio-based alternatives to underpin future global economies. Single carbon feedstocks, derived from CO2, represent promising substrates for biotechnological applications. Especially methanol is gaining prominence for bio-production of commodity chemicals.
Results In this study, we show the potential of Komagataella phaffii as a production platform for itaconic acid using methanol as the carbon source. Successful integration of heterologous genes from Aspergillus terreus ( cadA , mttA and mfsA ) alongside fine-tuning of the mfsA gene expression, led to promising initial itaconic acid titers of 28 g·L-1 after five days of fed-batch cultivation. Through the combined efforts of process optimization and strain engineering strategies we further boosted the itaconic acid production reaching titers of 55 g·L-1 after less than five days of methanol feed, whilst increasing the product yield on methanol from 0.06 g·g- 1 to 0.24 g·g-1.
Conclusion Our results highlight the potential of K. phaffii as a methanol-based platform organism for sustainable biochemical production.
### Competing Interest Statement
The authors have declared no competing interest.
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