Crystallization of SARS-CoV-2 nsp15 NendoU v1

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). The NSP15 endoribonuclease enzyme, known as NendoU, is highly conserved and plays a critical role in the ability of the virus to evade the immune system. NendoU is a promising target for the development of new antiviral drugs. This protocol was used to grow SARS CoV-2 NSP15 crystals that were applied high-throughput crystallographic fragment screening at XChem.