Activation of RIG-I signaling in the early stage of Paragonimus proliferus infection causes lung injury via type I immune response in rat

Paragonimiasis is a common zoonotic parasitic disease. The retinoic acid -inducible gene I (RIG -I) signaling is very important for the host to recognize invading pathogens (especially viruses and bacteria). However, the role of RIG -I signaling in the early stages of P. proliferus infection remains unclear. Therefore, in this study, Sprague-Dawley (SD) rat models with lung damage caused by P. proliferus were established. Experimental methods including Enzyme -linked Immuno Sorbent Assay (ELISA), real-time fluorescent quantitative polymerase chain reaction (PCR), western blotting, and hematoxylin and eosin (HE) staining were used to explore the mechanisms of lung injury caused by P. proliferus. As a result, the expression of the mRNA and proteins of RIG -I signal -related key target molecules, including RIG -I, tumor necrosis factor (TNF) receptor associated factor 6 (TRAF6), interferon regulatory Factor 7 (IRF7), IPS-1, and downstream C -X-C chemokine ligand 10 (CXCL10), were significantly up -regulated immediately after infection, peaked at 3 or 7 days, and showed a downward trend on after 14 days. The levels of pro -inflammatory cytokines interleukin-1 (IL -1), interferon (IFN)-alpha, -beta, and -gamma, which represent type 1 immune response, gradually increased and reached a peak by 14 days, which was consistent with the changes in the degree of inflammatory damage observed under HE staining of lung tissues. In conclusion, RIG -I signaling is activated in the early stage (before 14 days) of P. proliferus infection, it is inferred that the lung injury of the host may be related to the activation of RIG -I like signaling to induce type I immune response.