Figure 5 from Noninvasive Stratification of Colon Cancer by Multiplex PET Imaging

PET imaging phenotypic difference between primary and metastatic tumors. A, The generation of the Kras^G12D/+ Trp53^fl/fl Rosa26^N1icd/+ (KPN) and KPN liver metastasis organoid lines and subsequent implantation. One pair of lines, generated from a matched mouse primary tumor and liver metastasis, which were then propagated and injected subcutaneously into recipient mice (n = 5). B, Transverse and coronal PET/MRI slice images showing uptake of four PET tracers ([¹⁸F]FDG, [¹⁸F]FET, [¹⁸F]FLT, [¹⁸F]ACE) in subcutaneously implanted KPN primary and KPN liver metastasis organoids. KPN primary tumor-bearing mice are the same four PET ([¹⁸F]FDG, [¹⁸F]FET, [¹⁸F]FLT, [¹⁸F]ACE) images (primary) as displayed in Figs. 2B and 4B and D. C, Standard uptake peak values (SUV_peak) PET quantification from images in B. Sample size (n) is displayed on the bars. Error bars represent SD. Data compared using unpaired t test. Details of all mice used in these studies are presented in Supplementary Table S1. D, Representative GLUT-1 IHC and Lat-1/Slc7a5 ISH. Black scale bars represent 50 μm (*, P < 0.05; ***, P < 0.001; see also Supplementary Fig. S8). (A, Created with BioRender.com.)