Concentration, extraction and quantification of SARS-CoV-2 in wastewater via droplet digital PCR v2

This protocol describes the method used at Colorado State University to extract and quantify SARS-CoV-2 in wastewater collected from sewers and at wastewater treatment plants. The method relies on concentration via ultrafiltration, silica column based purification, and quantification via digital droplet reverse transcription PCR. Endogenous and exogenous controls are described.