FIGURE 3 from Low Exposures to Amphibole or Serpentine Asbestos in Germline <i>Bap1</i>-mutant Mice Induce Mesothelioma Characterized by an Immunosuppressive Tumor Microenvironment

Quantification of M2 macrophages in the TIME of asbestos-induced MMs from Bap1^+/− mice using an Aperio scanner, and IHC depicting arginase and iNOS staining of MM from asbestos-treated Bap1^+/− mice. A, Dot plot showing percentages of CD206-stained (left) and CD163-stained (center) cells in representative histologic images from crocidolite-induced and chrysotile-induced MMs from Bap1^+/− mice. Right, CD163 IHC staining of representative MMs from crocidolite-induced and chrysotile-induced MMs. Arrows indicate examples of cells positively stained for CD163. B, Serial sections of a crocidolite-induced and chrysotile-induced MMs from Bap1^+/− mice stained for arginase (Arg-1) and iNOS. Arrows indicate Arg-1-stained M2 macrophages.