Supplementary Figure S5 from Neutrophils Mediate Protection Against Colitis and Carcinogenesis by Controlling Bacterial Invasion and IL22 Production by γδ T Cells

Figure S5. γδ T cell subsets activation in DSS-treated mice. Related to Figure 4-5 A) Representative gating strategy for γδ T cell subsets in colon LP. B) Frequency of γδ T cell subsets from colon tissue of DSS-treated mice in colon LP. C) AhR expression in γδ T cells subset from colon LP of DSS-treated mice. D-E) Expression of IL-22 by γδ T cell subsets stimulated 4 hours with IL-23 plus IL-1β (D) and PMA plus ionomycin (E) analyzed by flow cytometry. F-G) Expression of IL-17 by γδ T cell subsets stimulated 4 hours with IL-23 plus IL-1β (F) and PMA plus ionomycin (G) analyzed by flow cytometry. H) RorγT expression in γδ T cell subsets from colon LP of DSS-treated mice. I) Body weight loss during DSS-induced acute colitis in Csf3r+/+(n=4), Csf3r-/- (n=4) and Csf3r-/- mice upon adoptive transfer of neutrophils (NΦ) (n=4) with and without antibiotic (ABX) oral treatment (Csf3r+/++ABX n=4 and Csf3r-/-+ABX n=4, Csf3r-/-+NΦ+ABX n=4). Red arrows indicated days of neutrophils transfer. A-H) Representative data of two independent experiments, Csf3r+/+(n=4), Csf3r-/- (n=4). I) One experiments. B-H) Multiple t-test. I) Wilcoxon matched-pairs signed rank test. Data are mean ± SEM. *** p < 0.001 ** p < 0.01 * p < 0.05.