Unveiling the Medicinal Power of Citrus limon Essential Oil: A Comprehensive Exploration of Antioxidant and Antimitotic Properties, Phytochemical Composition, and Molecular Inter-Actions

Background: The essential oil (EO) extracted from Citrus limon has gained attention for its potential bioactive properties due to its promising chemical composition. This study aimed to comprehensively evaluate the antioxidant and antimitotic activities of Methods: Volatile compounds were thoroughly analyzed using headspace and gas chromatography techniques. Molecular docking studies identified D-limonene and alpha-bergamotene as having the highest antioxidant potential. Assessment of antioxidant capacity employed 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ,3-Carotene bleaching assays, alongside the Total Antioxidant Capacity (TAC) assay. Absorption, distribution, metabolism, excretion and toxicity (ADMET) tests were conducted to confirm the EO's suitability for pharmaceutical applications. The antimitotic activity was evaluated using the Lepidium sativum test. Results: Results indicated a 1.99% essential oil yield, with D-limonene identified as the predominant constituent. In the DPPH assay, the half-maximal inhibitory concentration (IC50) value was 1.27 +/- 0.03 mg/mL, and the ,3-carotene bleaching assay exhibited an IC50 value of 6.74 +/- 0.12 mg/mL. The TAC assay determined the EO's total antioxidant capacity as 212.2 +/- 1.35 mu g Ascorbic acid (AA) eq/mg EO. The essential oil demonstrated significant antimitotic activity, reaching a maximum of 83.33% at a concentration of 250 mu g/mL. Conclusions: These findings underscore the noteworthy antioxidant and antimitotic capabilities of Citrus limon essential oil, primarily attributed to D-limonene and other bioactive components.