Steric hindrance of N6-methyl in m6A and its application for specific loci detection.
Chemical communications (Cambridge, England)(2024)
摘要
We found that the N6 methyl group of N6-methyladenine is able to hinder the methylation of N6-methyladenine at the N1 position by DMS. Based on this, we have devised a novel method for detecting N6-methyladenine, which was successfully applied to identify specific m6A loci in 28S rRNA.
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