Figure S1 from Lactate Utilization Enables Metabolic Escape to Confer Resistance to BET Inhibition in Acute Myeloid Leukemia

Figure S1: Glutaminolysis and fatty acid oxidation does not allow MOLM-13 to metabolically bypass BET inhibition. (A) MV-4-11 and MOLM-13 cells were cultured for 72 hr with BETi (0.15 µM) and viability quantified using a fluorescent plate reader. Viability was quantified relative to cells treated with vehicle control. Each point represents the mean (technical triplicate) value obtained from cells from an individual experiment (n=3). (B-E) MV-4-11 and MOLM-13 cells were loaded with a CellTrace dye and cultured for 72 hr with BETi and either (B-C) V-0302 (25 µM) or (D-E) etomoxir (150 µM). (B, D) To assess replication, CellTrace fluorescence intensity was quantified by flow cytometry and (C, E) viability was quantified in a fluorescent plate reader. Replication and viability were quantified relative to cells treated with vehicle control. (F) Raw CT values from qPCR experiment in Fig. 2A. Each point represents (B, D) the median fluorescent intensity (MFI) from or (C, E) the mean (technical triplicate) value obtained from cells from an individual experiment (n=3). (A) Paired t test or (B-E) Two-way ANOVA with Sidak’s multiple comparisons test (**P ≤ 0.01, ns = not significant).