Centella asiatica neuroprotective effect on 6-ohda-stimulated oxidative stress in differentiated sh-sy5y cells

Aim: Parkinson's disease is qualified by advancing the loss of dopaminergic neurons and depletion of dopamine. However, the pathophysiological mechanisms need new perspectives for therapeutic strategies that alleviate and abolish neurodegenerative. The last searches have demonstrated that Centella Asiatica is commonly used in conventional medicine. The aim of our study is to reveal the neuroprotective effect of Centella Asiatica, which we will use in the treatment, on the neurotoxicity stimulated by 6 OHDA. Method: First, the SH-SY5Y cell line was grown in prepared media. Then, 25-50-75 and 100 µg/ml concentrations of Centella Asiatica were supplemented to the wells that reached 85% confluence 2 hours before (except for the control and 6-OHDA groups). Afterward, 200 µM 6-OHDA was added to the wells (except for the control group) and incubated for 24 hours. Then, IL-1β, GSH, MTT, LDH, GPx, TNF-α, SOD, MPO, CAT, and MDA analyses were performed. One-way analysis of variance was performed using the IBM SPSS 22.0 package program. The results were compared with the control and 6-OHDA groups, and values below p<.05 were considered statistically significant. Results: It was found that Centella Asiatica demonstrated a dose-dependent rise in the vivacity rate and the cell vivacity was 92% at the highest concentration. Moreover, antioxidant parameters (GSH, GPx, SOD, CAT) correlated with MTT and LDH assay. In IL-1β, TNF-α, MPO, and MDA activities, it was observed that the oxidant amount reduced depending on the concentration. Conclusion: These findings revealed that Centella Asiatica exerts a neuroprotective effect opposite 6-OHDA induction by rising cell viability and reducing oxidative stress.