Large volume viral RNA extraction using MagMAX Viral RNA Isolation Kit v1

This method describes large volume nucleic acid purification from sewage concentrates starting with 1.2 mL of sample using MagMAX ‱ Viral RNA Isolation Kit. To perform automated extraction, follow steps in Workflow A, and for manual extractions follow Workflow B diverging after the initial sample processing with Lysis/Binding solution. Appendix 1 contains pictures providing a visual guide to manual magnetic beads based nucleic acid extraction.