Abstract 3383: Growth suppression and selective disruption of actin by alpha-santalol in human melanoma SK-MEL2 cells

Abstract Alpha-santalol, a major component of sandalwood oil has been shown to have chemopreventive and anti-tumor effects in different pre-clinical cancer models. The present study was undertaken to determine the in vitro efficacy of alpha-santalol on SK-MEL2 human melanoma cells and an immortalized human keratinocyte cell line (HACaT). In this study, we employed MTT assay, Trypan blue assay, wound healing assay, and confocal microscopy for imaging phalloidin/DAPI stained cells to investigate the cytotoxicity, cell viability, migratory potential respectively of the cells treated with different concentrations of alpha-santalol and DMSO for given time periods. Results showed that alpha-santalol treatment significantly decreased SK-MEL2 cell viability, wound healing, while only affecting HACaT cells at higher concentrations. Alpha-santalol treatment also disrupted actin cytoskeleton in SK-MEL2 cells, whereas HACaT cells were more resistant to this effect. In conclusion, the present study reveals selective growth inhibitory and anti-migratory effects of alpha-santalol in human melanoma cells and warrants future studies to systemically explore the mechanistic details involved in its growth inhibitory and antimigratory effects. Citation Format: Ajay Bommareddy, Ritesh Chandrasekaran, Michael Lu, Delilah Penate. Growth suppression and selective disruption of actin by alpha-santalol in human melanoma SK-MEL2 cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 3383.