CRISPR/Cas9-Mediated Knockdown in LUHMES Cells: Nucleofection and Validation Protocol v3

Utilizing a CRISPR RNP complex and nucleofection, this protocol enables precise gene knockout. LUHMES cells, sourced from human fetal mesencephalic tissue, provide a valuable model for investigating dopaminergic dysfunction. They exhibit stimulus-induced dopamine release, pertinent electrophysiological traits, and distinctive dopaminergic markers, validating their phenotypic relevance. LUHMES Cell Line: (ATCC Catalog Number: CRL-2927) Nucleofection kit: P3 Primary Cell 4D-Nucleofector X Kit S Catalog #V4XP-3032