FIGURE 4 from Immune Profiling of Vulvar Squamous Cell Cancer Discovers a Macrophage-rich Subtype Associated with Poor Prognosis

CD163⁺ TAMs are associated with lymphogenesis. A, LVD and LVI were assessed in the stroma using D2-40–stained lymphatic endothelium. Intratumoral and stromal CD163 immunoreactivity was recorded as in Fig. 1B; data are presented as mean immunoreactivity normalized to tissue compartments in percent. CD163⁺ TAMs were categorized on the basis of high/low LVD and the presence/absence of LVI. Individual datapoints, shown as dots, overlap summary statistics boxplots with medians represented by horizontal center lines. Significance analysis by two-sided Mann–Whitney U test with Benjamini–Hochberg procedure. B, BVD and BVI were assessed in the stroma using CD31-stained vessels. Intratumoral and stromal CD163 immunoreactivity was recorded as in Fig. 1B; data are presented as mean immunoreactivity normalized to tissue compartments in percent. CD163⁺ TAMs were categorized on the basis of high/low BVD and the presence/absence of BVI. Individual datapoints, shown as dots, overlap summary statistics boxplots with medians represented by horizontal center lines. Significance analysis by two-sided Mann–Whitney U test with Benjamini–Hochberg procedure.