Understanding systems level metabolic adaptation resulting from osmotic stress

An organism’s survival hinges on maintaining the right thermodynamic conditions. Osmotic constraints limit the concentration range of metabolites, affecting essential cellular pathways. Despite extensive research on osmotic stress and growth, understanding remains limited, especially in hypo-osmotic environments. To delve into this, we developed a novel modeling approach that considers metabolic fluxes and metabolite concentrations along with thermodynamics. Our analysis of E. coli adaptation reveals insights into growth rates, metabolic pathways, and thermodynamic bottlenecks during transitions between hypo- and hyper-osmotic conditions. Both experimental and computational findings show that cells prioritize pathways that have higher thermodynamic driving force, like the pentose phosphate or the Entner–Doudoroff pathway, under low osmolarity. This work offers a systematic and mechanistic explanation for reduced growth rates in hypo- and hyper-osmotic conditions. The developed framework is the first of its kind to incorporate genome wide constraints that consider both natural logarithm and actual metabolite concentrations. ![Figure][1] ### Competing Interest Statement The authors have declared no competing interest. [1]: pending:yes