FIGURE 5 from Activity of the Ubiquitin-activating Enzyme Inhibitor TAK-243 in Adrenocortical Carcinoma Cell Lines, Patient-derived Organoids, and Murine Xenografts

Synergy between TAK-243 and mitotane. A, Top: The indicated ACC cell lines were treated with TAK-243 with or without mitotane for 72 hours. Bottom: CI values were calculated and plotted. B, Enhanced induction of apoptosis by the TAK-243 + mitotane combination was measured as caspase-3 cleavage. C, Enhanced UPR induction by the TAK-243 + mitotane combination. CU-ACC2 cells were treated with or without mitotane (40 µmol/L) for 24 hours before adding the indicated concentrations of TAK-243 for 4 hours. UPR proteins were evaluated by Western blotting.