Non-invasive laser speckle imaging of extra-embryonic blood vessels in intact few-days-old avian eggs

Zhenyu Dong,Simon Mahler, Carol Readhead, Xi Chen, Maya Dickson,Marianne E Bronner,Changhuei Yang

biorxiv(2024)

引用 0|浏览1
暂无评分
摘要
Imaging blood vessels in early-stage avian embryos has a wide range of practical applications for developmental biology studies, drug and vaccine testing, and early sex determination. Optical imaging such as brightfield transmission imaging offers a compelling solution due to its safe non-ionizing radiation, and operational benefits. However, it comes with challenges such as eggshell opacity and light scattering. To address these, we have revisited an approach based on laser speckle contrast imaging (LSCI) and demonstrated a high quality, comprehensive and non-invasive visualization of blood vessels in few-days-old chicken eggs, with blood vessel as small as 100 um in diameter (with LSCI profile full-width-at-half-maximum of 275 um). We present its non-invasive use for monitoring blood flow, measuring the embryo's heartbeat, and determining the embryo's developmental stages using machine learning with 85% accuracy from stage HH15 to HH22. This method can potentially be used for non-invasive longitudinal studies of cardiovascular development and angiogenesis, as well as egg screening for the poultry industry. ### Competing Interest Statement The authors have declared no competing interest.
更多
查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要