Aspirin-Mediated Acetylation of SIRT1 Maintains Intestinal Immune Homeostasis

Aspirin, also named acetylsalicylate, can directly acetylate the side-chain of lysine in protein, which leads to the possibility of unexplained drug effects. Here, the study used isotopic-labeling aspirin-d3 with mass spectrometry analysis to discover that aspirin directly acetylates 10 HDACs proteins, including SIRT1, the most studied NAD+-dependent deacetylase. SIRT1 is also acetylated by aspirin in vitro. It is also identified that aspirin directly acetylates lysine 408 of SIRT1, which abolishes SIRT1 deacetylation activity by impairing the substrates binding affinity. Interestingly, the lysine 408 of SIRT1 can be acetylated by CBP acetyltransferase in cells without aspirin supplement. Aspirin can inhibit SIRT1 to increase the levels of acetylated p53 and promote p53-dependent apoptosis. Moreover, the knock-in mice of the acetylation-mimic mutant of SIRT1 show the decreased production of pro-inflammatory cytokines and maintain intestinal immune homeostasis. The study indicates the importance of the acetylated internal functional site of SIRT1 in maintaining intestinal immune homeostasis. Using isotopic labeling aspirin-d3 and mass spectrometry analysis, aspirin directly acetylates 10 HDAC proteins, including SIRT1, a well-studied NAD+-dependent deacetylase. Aspirin acetylates lysine 408 of SIRT1, inhibiting its deacetylase activity and impairing substrate binding. This inhibition promotes p53-dependent apoptosis and, in knock-in mice with the acetylation-mimic mutant of SIRT1, helps maintain intestinal immune homeostasis by reducing pro-inflammatory cytokine production. image