Ocean liming in the oligotrophic Eastern Mediterranean: impact on the planktonic microbial food web

Ocean Alkalinity Enhancement (OAE) allows for active removal of atmospheric CO2, therefore is considered as one of the most promising Carbon Dioxide Removal (CDR) technologies. OAE could be obtained by discharging alkaline material in the wake of ships, however very little is known on potential negative effects on marine communities. We report here the first study focusing on the response of the entire pelagic microbial food web to the addition of calcium hydroxide in real oligotrophic conditions. In a mesocosm experiment performed at the CretaCosmos facility in Crete, Greece, in May-June 2023, we tested the response of the eastern Mediterranean oligotrophic waters to two different treatments of calcium hydroxide slurry addition (SL; High and Low concentrations, three replicate mesocosms each), while three more mesocosms served as Controls (no addition). Mesocosms, filled with natural coastal seawater, were treated with slurry on days 1, 3, 5, 7, 9, 11 to simulate the chronic disturbance, expected from repeated discharge of SL from ships; while the possible precipitation of carbonate crystals was assessed by putting a sediment trap at the bottom of each mesocosm. The carbonate-equilibrium and dissolution-kinetics were monitored by measuring temperature, solution-conductivity, and changes in pH. Photosynthetically-Active-Radiation and visible light were monitored by sensors in each mesocosm. Plankton productions (bacterial, viral, secondary) as well as community composition of all plankton groups from viruses to copepods were assessed by optical microscopy, flow cytometry and metagenomics; chlorophyll was also measured. Although an important alteration of pH was observed in the High lime addition, only heterotrophic bacteria production was found to be negatively affected and only in the second half of the experiment. The rest of the plankton groups presented different patterns and not a clear response to the lime addition. This first attempt to study the effect of lime addition on the complex pelagic food web will serve as a first step to an extensive testing needed before any application of ocean liming at a large scale.