The effect of changes in morphology and structure of ZnS particle spheres on H2S gas release and cellular uptake behavior

Because preparation circumstances are limited, the relationship between spherical porous zinc sulfide (ZnS) and cells is rarely investigated. Thus, using the hydrothermal approach, four different types of ZnS spheres with consistent size, high dispersion, and stability were created in this research by varying the temperature, duration, solvent ratio, and pH level of the hydrothermal process. Three structures are present in the generated particle spheres: solid, hollow, and mesoporous structures. In addition to causing a significant variation in the particle spheres' specific surface area, porosity, and surface roughness, the distinct architectures also have an impact on ZnS particle spheres' capacity to produce hydrogen sulfide (H2S) gas in an acidic environment. Experiments on hemolysis and cytotoxicity demonstrated that ZnS particle spheres with varying concentrations and shapes exhibited no cytotoxicity and good biocompatibility. According to cellular uptake trials, ZnS particle spheres with a rougher surface structure and a bigger specific surface area are more likely to be absorbed by macrophages and release more H2S gas. This activity of releasing H2S gas by H+ action and MA phagocytosis by employing ZnS as a donor lays the groundwork for later particles to load medicines and carry out biological functions.