Extraction and Characterization of Collagen Hydrolysate from Buffalo (Bubalus Bubalis) Skin Including its Antioxidant Properties and Antiarthritic Effect

This study was conducted to characterize the extract collagen hydrolysate (CH) from low value buffalo skin using enzymes bromelain (B) and papain (P), including their bioactivities. Optimum levels of the two enzymes were determined by Sodium dodecyl sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE) and degree of hydrolysis. Levels (30 and 50 units of B/g of skin and 20 and 30 units of P/g of skin) showing maximum degradation of collagen proteins were used to extract CH and the recovered CH were correspondingly referred as B20, B50, P20 and P30, respectively. The yield of CH from skin for P20, P30, B30 and B50 was 27.38, 26.32, 20.71 and 16.19