Electrospun statin-loaded nanofibers for treating diabetic wounds

This study aims to develop, characterize, and evaluate the best statin-loaded Collagen Silver nanoparticle-coated polycaprolactone nanofibers by electrospinning technique. The characterization and evaluation results reveal that nanofibers had shown an aligned Collagen Silver nanoparticle-coated polycaprolactone nanofibers distribution of fibers and aliment of collagen and silver nanoparticle coating material on the surface of the nanofibers. Furthermore, the electrospun nanofibers have shown no interaction between the drug and collagen and PCL polymeric material and have demonstrated controlled drug release for 360 h and significant degradation for 60 days of the study. In vitro, among the nanofiber treatment groups and the control groups, Pravastatin Collagen Silver nanoparticle-coated polycaprolactone nanofibers have shown cell proliferation and migration of NHDF cells and blood vessel development in the CAM assay and potential anti-bacterial activity against S. aureus and E. coli. In vivo, the Pravastatin Collagen Silver nanoparticle-coated polycaprolactone nanofibers have demonstrated improved wound recovery, re-epithelization, fibroblast cell proliferation, angiogenesis, and ECM remodeling and enhanced VEGF-sprouting in the excised tissues of streptozotocin-induced diabetic rats for 7, 14, and 21 days of the study. Therefore, the developed Pravastatin Collagen Silver nanoparticle-coated polycaprolactone nanofibers could be a promising wound-dressing platform for effectively treating and managing diabetic wounds.Highlights Nanofiber wound dressings provide numerous structural features in biomaterials. Polycaprolactone can release the repurposed statin molecules into the wound site in a controlled manner. In diabetic wounds, ECM tissue remodeling stimulates repair and vessel growth. Pravastatin has been demonstrated to improve angiogenesis and ECM secretion for wound healing in diabetic conditions. Electrospun nanofiber wound dressings provide numerous appealing structural features in the field of biomaterials, such as extracellular matrix (ECM)-like architecture, large surface-to-volume ratio, and programmable surface structure, which have lately rekindled major interest in skin tissue regeneration. The selection of polycaprolactone, a stable polymeric matrix system, can release the repurposed statin molecules into the wound site in a controlled manner. In diabetic wounds, angiogenesis and ECM tissue remodeling are critical steps that regenerate blood vessels and enable the oxygen supply necessary to stimulate repair and vessel growth. However, decreased secretion of angiogenic factors and the migration or proliferation of fibroblast and endothelial cells delay the healing of diabetic wounds. Hence, Pravastatin has improved angiogenesis and ECM secretion in the present work by upregulating essential growth factors necessary for wound healing in diabetic conditions. image