Enhancing biological properties with straightforward deposition of durable heparin/chitosan surface coatings on wettable poly(ε-caprolactone)/Tween-20 electrospun fibers

Poly(ε-caprolactone) (PCL) is a hydrophobic aliphatic polyester commonly used as a biomaterial due to its cytocompatibility and biodegradability. However, PCL-based materials are hydrophobic and susceptible to bacterial adhesion and proliferation. To address these disadvantages, this study proposes a direct and practical method for functionalizing the surface and enhancing the biological properties of electrospun PCL fibers using Tween-20 surfactant, followed by the deposition of polyelectrolyte multilayers (PEMs). Tween-20, a neutral and cytocompatible surfactant, was directly incorporated into the fibers without prior surface modifications. The surfactant significantly increased the wettability of the PCL fibers, resulting in water contact angles of 0 degrees, facilitating subsequent functionalization with heparin and chitosan PEMs (with 10, 20, and 30 layers). The PEMs were characterized through scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), water contact angle and mechanical measurements, Fourier transform infrared-attenuated total reflectance (FTIR-ATR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). The PEMs remain stable on the fiber surfaces for up to 14 days in a buffer at 37°C. The samples supported high cell viability with no observed cytotoxicity toward human adipose-derived stem cells and no hemolytic activity toward human red blood cells. Antibacterial assays (live/dead and proliferation tests) demonstrated that the coated PCL fibers inhibited the proliferation of Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). The PEM coatings significantly enhanced the antimicrobial activity, resulting in a high number of damaged and dead bacterial cells attached to the coated fiber. Therefore, the heparin/chitosan PEMs on Tween-20-modified PCL fiber combat bacterial infection while supporting mammalian cells.