Spectra-separated depth-of-field extended fluorescence imaging through scattering media using speckle deconvolution

•Fluorescence imaging through a thin scattering media using speckle deconvolution.•No PSF pre-characterization is required with dual-color fluorescence, which enables speckle deconvolution in relatively simple and non-invasive manner.•Extending the depth-of-field of scattering imaging system by PSF manipulation.