Crystal structure of HPPD inhibitor sensitive protein from Oryza sativa
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS(2024)
摘要
4-hydroxyphenylpyruvate dioxygenase (HPPD) Inhibitor Sensitive 1 (HIS1) is an endogenous gene of rice, conferring broad-spectrum resistance to 8-triketone herbicides. Similar genes, known as HIS1-like genes (HSLs), exhibit analogous functions and can complement the herbicide-resistant characteristics endowed by HIS1. The identification of HIS1 and HSLs represents a valuable asset, as the intentional pairing of herbicides with resistance genes emerges as an effective strategy for crop breeding. Encoded by HIS1 is a Fe(II)/2-oxoglutaratedependent oxygenase responsible for detoxifying 8-triketone herbicides through hydroxylation. However, the precise structure supporting this function remains unclear. This work, which determined the crystal structure of HIS1, reveals a conserved core motif of Fe(II)/2-oxoglutarate-dependent oxygenase and pinpoints the crucial residue dictating substrate preference between HIS1 and HSL.
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关键词
HPPD inhibitor sensitive 1 (HIS1),HIS1-like (HSL) genes,8-triketone herbicide,Mutation,X-ray crystallography
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