In vivo mapping of rat brain partial white matter content using hexachlorophene-induced neurotoxicity model

Segmentation of the white matter in MRI scans of the rat brain presents a significant challenge due to the low contrast. Existing anatomical reference maps of rat brain are usually constructed from fixed tissue, which may suffer from geometrical distortions due to fixation/processing. To significantly increase the in vivo contrast between white and gray matter in the rat brain we used a known neurotoxicant hexachlorophene, which produces selective white matter damage. This model was used to map white matter in the rat brain and estimate the partial white matter content in any given imaging voxel. Hexachlorophene was administered to rats at a dose of 30 mg/kg orally once a day over five consecutive days. A significant white matter changes were observed using quantitative T2 maps, from which the partial white matter content throughout the whole rat brain was derived. Several assumptions were made: hexachlorophene affects T2 relaxation only in the white matter; T2 of gray matter is relatively uniform in the brain; apparent T2 value in a given voxel is a combination of T2s from white and gray matter portions of that voxel, hexachlorophene affects nearly 100 % of white matter. The partial white matter map of the rat brain was constructed with the resolution of 0.2 x 0.2 x 1.0 mm per voxel. This map could be adjusted for segmentation of the brain tissue with preset threshold of the white matter content, or to establish the tissue composition in any region of interest among other applications.