A multifunctional organophosphonic ligand-templated poly(polyoxotungstate) with potential in building a genosensor for thalassemia gene detection

A novel PV-SbIII heteroatom-inserted poly(polyoxotungstate) (poly(POT)) [H2N(CH3)2]5Na11H9[(HEDATMPA)SbW15O50]{[PrW4O10][B-alpha-SbW9O33]2}center dot 38H2O (1) was synthesized and is composed of an attractive [(HEDATMPA)SbW15O50]14- hybrid fragment and one sandwich-like PrIII-inserted [PrW4O10(B-alpha-SbW9O33)2]11- entity by using ethylenediamine tetramethylphosphoric acid (H8EDATMPA) with potential heteroatom sites and functional organic groups. Considering the advantages of poly(POT) clusters in storing and transferring electrons, we fabricated a 1-PEDOT (PEDOT = poly(3,4-ethylenedioxythiophene)) hybrid film, which was used as an electrode-modified material to construct a 1-based genosensor (GS) for detecting the beta-thalassemia gene. Research results show that this 1-based GS demonstrates high specificity, outstanding antijamming ability and low detection limit for beta-thalassemia gene detection. This work not only provides a novel and feasible preparative strategy to construct poly(POT) clusters, but also promisingly promotes the development of poly(POT)-based GSs. An organophosphonic ligand-templated PV-SbIII double-heteroatom-intercalated polyoxotungstate 1 was synthesized and the ability of the 1-based genosensor to detect the beta-thalassemia gene was evaluated.