Comparison of DNA extraction modification using QIAamp DNA mini kit vs boiling methods for M. Tuberculosis isolate

Background: The innovation of commercial DNA extraction kits and the application of molecular technology have recently been utilized in our understanding of microbial genomics. DNA extraction is a crucial step for molecular methods like Polymerase Chain Reaction. This study aimed to compare the modification of QIAamp DNA mini kit and a cheap, rapid, noncommercial method, the boiling method for extraction of Mycobacterium tuberculosis (M.tb) isolate.Methods: Both experimental protocols were applied to ten mycobacterial isolates from TB patients. The extracted DNA's purity and concentration with a spectrophotometer were evaluated, continued by electrophoresis to ensure the DNA's integrity.Results: The process of boiling was shown to be more effective, easy, affordable, and acceptable for all of these isolates. The mean purity of DNA A260/280 by modification of the QIAamp DNA mini kit and boiling were 1.48 and 1.76. And the DNA yield were 22.8 and 116.39 ug/ml respectively.Conclusion: Boiling method for M.tb isolates DNA extraction is a promising method that should be further explored and validated.