Synthesis and anticancer mechanism of ferroptosis of binuclear Os(II)-arene complex

Here, we describe [Os(eta(6)-bip)(1,3-bib)Cl](2),Cl-2 (bib-Os) (eta(6)-bip=eta(6)-biphenyl,1,3-bib=1,3-di(1H-imidazol-l-y1) benzene), a binuclear Os(II) complex, which was characterized by H-1 NMR and ESI-MS. The results showed that complex bib-Os had good lipophilieiiy (1g P-u/w=1.52) and was easy to accumulate in cells. Complex bib-Os showed high antiproliferative activity against human ovarian A2780 cancer cells (IC50 =4.2 mu mol center dot L-1), producing a large number of reactive oxygen species (ROS) and inducing mitmhondrial morphological damage and membrane potential decline. Lipid peroxide (LPO) accumulation, glutathione (GSH) depletion, and glutathione peroxidase 4 (GPX4) inhibition further verified bib-Os-induced cell death through the ferroptosis pathway.