Microbial production and applications of -glucosidase-A review

beta-Glucosidase exists in all areas of living organisms, and microbial beta-glucosidase has become the main source of its production because of its unique physicochemical properties and the advantages of high-yield production by fermentation. With the rise of the green circular economy, the production of enzymes through the fermentation of waste as the substrate has become a popular trend. Lignocellulosic biomass is an easily accessible and sustainable feedstock that exists in nature, and the production of biofuels from lignocellulosic biomass requires the involvement of beta-glucosidase. This review proposes ways to improve beta-glucosidase yield and catalytic efficiency. Optimization of growth conditions and purification strategies of enzymes can increase enzyme yield, and enzyme immobilization, genetic engineering, protein engineering, and whole-cell catalysis provide solutions to enhance the catalytic efficiency and activity of beta-glucosidase. Besides, the diversified industrial applications, challenges and prospects of beta-glucosidase are also described.