Simultaneous detection of 22 mycotoxins in grape by QuEChERS and --ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap -high-resolution mass spectrometry

To effectively assess the extent of the mycotoxin contamination of grapes, a method was developed to analyze 22 mycotoxins by ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap highresolution mass spectrometry (UPLC-Q-Orbitrap .HRMS). Samples were extracted and purified using the QuEChERS (quick, easy, cheap, effective, rugged, and safe) .procedure. The extracts were separated on a BEH Shield C18 column (100 mm x .2.1 mm, .1.7 mu m), using methanol and water with .0.1% formic acid solution and 2 mmol/L ammonium acetate as the mobile .phases. The quantification was performed according to a matrix-matched external standard .method. There was a good linear relationship within the respective mass concentration ranges of 22 mycotoxins; the correlation coefficients were not less than .0.998 .1. The limit of quantification was .-.0.3-10.0 .mu g/kg. Mycotoxin recovery ranged from .68.6% to .109.0%, with a relative standard deviation of .-..1.28%-12.80%. The method is simple, rapid, highly sensitive, and accurate, making it useful for screening grapes for common and emerging .mycotoxins.