Positive effects of plant diversity on dry matter yield while maintaining a high level of forage digestibility in intensively managed grasslands across two contrasting environments

The local abiotic and environmental conditions of a grass-based farming system may influence the agricultural benefits of mixtures in comparison to pure stands. We investigated the effects of species identities and interactions between grass, legume, and herb species on dry matter yield and sward digestibility and explored how contrasting environments may affect these relationships. We established experimental plots across 11 mixtures and 4 monocultures of perennial ryegrass (Lolium perenne L.), chicory (Cichorium intybus L.), white clover (Trifolium repens L.), and red clover (Trifolium pratense L.) established at two seeding densities in two sites Brody and Szelejewo (Poland) and managed them over a 3-year period. The two sites were close geographically and had similar climates, but differed in soil types (Luvisols and Cambisols, respectively). We confirmed that the annual DM yields were generally higher on Cambisols soil as compared to Luvisols soil; both individual species effects and species interaction strengths differed across the two contrasting environments. The predicted annual DM yield for the 4-species mixtures in general gave comparable or higher yields compared to monocultures across the 3 years, with the mixtures giving around 10%-30% higher yields than the weighted average of the monocultures of the constituent species. Analysing the transgressive overyielding it turned out that there was no significant difference between the 4-species mixtures and best performing monoculture. The benefits of species diversity in our study was greater on less productive site because we found larger overyielding of the 4-species sward on the Luvisols soil than on the Cambisols soil. Additionally, the effect of mixtures composed by 4-species increased the yield stability compared to monocultures in the 3-year period of our study, particularly on Cambisols soil. Sward digestibility applied to average values did not differ much between sites with mixtures performing similarly to monocultures. The reason for that could have been the dynamics of sward botanical composition during study years shifted towards increased perennial ryegrass and decreased proportions of chicory in the sward as well as the observed phenomenon that the species in mixed swards progressed to successive growth stages more slowly than in pure sowing.