Therapeutic Potential of Human Mesenchymal Stem Cells Engineered with Different Concentrations of TNFcr and IFNg

Delivered by Ingenta Aims: In this study, the authors aimed to examine the functional activities (adhesion, proliferation, and migration) of hMSCs under the effects of various concentrations of inflammatory mediators, such as TNFa or IFNg. Methods: HMSCs were exposed to various concentrations of the inflammatory mediators TNFa or IFNg, and the effects of TNFa or IFNg on the functional properties of hMSCs were determined using multiple functional assays (adhesion, proliferation, and migration). HMSC expression of genes (cytokine, chemokine, and adhesion molecule genes) was analyzed using real-time polymerase chain reaction (RT-PCR). Results: TNFa promoted their proliferation and migration especially at 100 ng/ml, while IFNg increased proliferation only at concentrations of 10 ng/ml and proliferation at 50 and 100 ng/ml. Conclusion: This study evaluated the suitability of hMSCs in treating inflammatory diseases, which are characterized by high levels of inflammatory mediators such as TNFa or IFNg, and proved that the treatment of hMSCs with TNFa or IFNg at higher concentrations has a protective effect.