CRISPR/CasΦ2-mediated gene editing in wheat and rye.

Sanzeng Zhao, Xueying Han, Yachen Zhu, Yuwei Han, Huiyun Liu,Zhen Chen, Huifang Li,Dan Wang, Chaofan Tian, Yuting Yuan,Yajie Guo,Xiaomin Si,Daowen Wang,Xiang Ji

Journal of integrative plant biology（2024）

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摘要

The compact CRISPR/CasΦ2 system provides a complementary genome engineering tool for efficient gene editing including cytosine and adenosine base editing in wheat and rye with high specificity, efficient use of the protospacer-adjacent motif TTN, and an alternative base-editing window.

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